FLAG-EglN2 H358A-pBabe-puro
(Plasmid #22705)

• Depositing Lab: William Kaelin
• Publication: Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Backbone

• Vector backbone: pBabe-Puro
• Backbone size w/o insert (bp): 5145
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha or XL1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Egg laying Nine - 2 (EglN2)
• Alt name: prolyl hydroxylase 1 (PHD1)
• Species: H. sapiens (human)
• Insert Size (bp): 1260
• Mutation: mutated cDNA: point mutation in codon 358 changing amino acid H358 to Alanine, rendering the encoded enzyme inactive
• GenBank ID: BC036051
• Entrez Gene: EGLN2 (a.k.a. EIT-6, EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: pBabe-5'
• 3′ sequencing primer: pBabe-3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry