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Addgene

eIscB
(Plasmid #227016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SV40 NLS-OgeuIscB-v3-SV40-nucleoplasmin NLS
  • Species
    Synthetic
  • Mutation
    D96R/E84R/V159R
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eIscB was a gift from Dali Li (Addgene plasmid # 227016 ; http://n2t.net/addgene:227016 ; RRID:Addgene_227016)

  • For your References section:

    Engineering IscB to develop highly efficient miniature editing tools in mammalian cells and embryos. Xue N, Hong D, Zhang D, Wang Q, Zhang S, Yang L, Chen X, Li Y, Han H, Hu C, Liu M, Song G, Guan Y, Wang L, Zhu Y, Li D. Mol Cell. 2024 Aug 22;84(16):3128-3140.e4. doi: 10.1016/j.molcel.2024.07.007. Epub 2024 Aug 2. 10.1016/j.molcel.2024.07.007 PubMed 39096898
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