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Addgene

pPerimCh
(Plasmid #227013)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFCcGi (Addgene #59324)
  • Backbone manufacturer
    Sophie Helaine, David Holden
  • Total vector size (bp) 7540
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PelB-mCherry, arabinose inducible cytosolic GFP
  • Species
    Synthetic
  • Insert Size (bp)
    3681
  • Promoter rpsM and pBAD

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Sophie Helaine & David Holden (Addgene plasmid # 59324) for the backbone plasmid.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPerimCh was a gift from Suzan Rooijakkers (Addgene plasmid # 227013 ; http://n2t.net/addgene:227013 ; RRID:Addgene_227013)

  • For your References section:

    Complement-dependent outer membrane perturbation sensitizes Gram-negative bacteria to Gram-positive specific antibiotics. Heesterbeek DAC, Martin NI, Velthuizen A, Duijst M, Ruyken M, Wubbolts R, Rooijakkers SHM, Bardoel BW. Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.1038/s41598-019-38577-9. 10.1038/s41598-019-38577-9 PubMed 30816122
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