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Addgene

pcDNA-Sin-Cas9
(Plasmid #227006)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227006 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    S. iniae
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG, SV40 NLS (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Sin-Cas9 was a gift from Charles Gersbach (Addgene plasmid # 227006 ; http://n2t.net/addgene:227006 ; RRID:Addgene_227006)

  • For your References section:

    Characterization of diverse Cas9 orthologs for genome and epigenome editing. Butterfield GL, Rohm D, Roberts A, Nethery MA, Rizzo AJ, Morone DJ, Garnier L, Iglesias N, Barrangou R, Gersbach CA. Proc Natl Acad Sci U S A. 2025 Mar 18;122(11):e2417674122. doi: 10.1073/pnas.2417674122. Epub 2025 Mar 12. 10.1073/pnas.2417674122 PubMed 40073054
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