MSCV P2Gm +SwaI
(Plasmid #22699)

• Depositing Lab: Richard Hynes
• Publication: MSCV P2Gm +SwaI (unpublished)

Backbone

• Vector backbone: MSCV2.2 with WPRE cloned into ClaI site is the base vector
• Backbone size w/o insert (bp): 7500
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: grow in stbl3 cells at 30oC
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miR30

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • FLIP vector protocols
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See FLIP vector protocols (PDF from this page) for more information.

This vector contains the SwaI site for cloning multiple shRNAs into a single vector.