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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)
(Plasmid #226989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BPK1520
  • Backbone manufacturer
    Keith Joung, Addgene plasmid #65777
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 gRNA 1a to create OPA1-del_exon5
  • Alt name
    CJT90
  • Alt name
    pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)
  • gRNA/shRNA sequence
    GCTCATTGTGAACTCGTGGCA
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer oS280-CAGGGTTATTGTCTCATGAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90) was a gift from Benjamin Kleinstiver (Addgene plasmid # 226989 ; http://n2t.net/addgene:226989 ; RRID:Addgene_226989)

  • For your References section:

    In situ architecture of Opa1-dependent mitochondrial cristae remodeling. Fry MY, Navarro PP, Hakim P, Ananda VY, Qin X, Landoni JC, Rath S, Inde Z, Lugo CM, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH. EMBO J. 2024 Feb;43(3):391-413. doi: 10.1038/s44318-024-00027-2. Epub 2024 Jan 15. 10.1038/s44318-024-00027-2 PubMed 38225406
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