WDV-Echistatin
(Plasmid #226985)

• Purpose: Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors
• Depositing Lab: Wendy Gordon
• Publication: Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi: 10.1038/s41467-023-38157-6.

Backbone

• Vector backbone: pTD68
• Backbone size w/o insert (bp): 5718
• Total vector size (bp): 6294
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: WDV
• Alt name: Wheat Dwarf Virus Rep Domain
• Insert Size (bp): 420
• Entrez Gene: repA (a.k.a. WDVgp4)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • SUMO (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Gene/Insert 2

• Gene/Insert name: Echistatin
• Alt name: Chain A, Disintegrin echistatin
• Species: E. Carinatus (Saw Scaled Viper)
• Insert Size (bp): 156
• GenBank ID:
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry