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Addgene

WDV
(Plasmid #226983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTD68
  • Backbone size w/o insert (bp) 5718
  • Total vector size (bp) 6141
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WDV
  • Alt name
    Wheat Dwarf Virus Rep Domain
  • Insert Size (bp)
    423
  • Entrez Gene
    repA (a.k.a. WDVgp4)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • SUMO (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WDV was a gift from Wendy Gordon (Addgene plasmid # 226983 ; http://n2t.net/addgene:226983 ; RRID:Addgene_226983)

  • For your References section:

    RAD-TGTs: high-throughput measurement of cellular mechanotype via rupture and delivery of DNA tension probes. Pawlak MR, Smiley AT, Ramirez MP, Kelly MD, Shamsan GA, Anderson SM, Smeester BA, Largaespada DA, Odde DJ, Gordon WR. Nat Commun. 2023 Apr 28;14(1):2468. doi: 10.1038/s41467-023-38157-6. 10.1038/s41467-023-38157-6 PubMed 37117218
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