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pHisrKr2
(Plasmid #22698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-16b
  • Backbone size w/o insert (bp) 5711
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha (normal growth conditions)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rat Kruppel-type protein 2
  • Alt name
    rKr2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    492
  • Entrez Gene
    Zfp111 (a.k.a. rKr2)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 terminal primer
  • 3′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Rat rKr2 DNA was cloned in Hudson laboratory

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pHisrKr2 was constructed to express a part of rKr2 as a histidine-tagged recombinant protein in E. coli for antibody production. This rKr2 DNA fragment encodes amino acids 48-211 of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHisrKr2 was a gift from Lynn Hudson (Addgene plasmid # 22698 ; http://n2t.net/addgene:22698 ; RRID:Addgene_22698)

  • For your References section:

    Expression of the Kruppel-type zinc finger protein rKr2 in the developing nervous system. Lovas G, Li W, Pott U, Verga T, Hudson LD. Glia. 2001 Apr 15. 34(2):110-20. 10.1002/glia.1046 PubMed 11307160
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