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Addgene

pET28a-OuIAD(H514A)
(Plasmid #226971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OuIAD(H514A)
  • Species
    Olsenella uli DSM 7084
  • Mutation
    H514A
  • Tag / Fusion Protein
    • His6 (on backbone) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-OuIAD(H514A) was a gift from Emily Balskus (Addgene plasmid # 226971 ; http://n2t.net/addgene:226971 ; RRID:Addgene_226971)

  • For your References section:

    Mechanistic Studies of a Skatole-Forming Glycyl Radical Enzyme Suggest Reaction Initiation via Hydrogen Atom Transfer. Fu B, Nazemi A, Levin BJ, Yang Z, Kulik HJ, Balskus EP. J Am Chem Soc. 2022 Jun 29;144(25):11110-11119. doi: 10.1021/jacs.1c13580. Epub 2022 Jun 15. 10.1021/jacs.1c13580 PubMed 35704859
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