G-CaMP3
(Plasmid #22692)

• Purpose: a single-wavelength GCaMP2-based genetically encoded calcium indicator
• Depositing Lab: Loren Looger
• Publication: Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: G-CaMP3
• Alt name: GCaMP3
• Insert Size (bp): 1300
• Mutation: N363D mutation (increased fluorescence)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 71 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few sequence differences between author sequence and Addgene quality control sequence. The function of the plasmid is not affected.