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Addgene

pAM251
(Plasmid #226895)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226895 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-DUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pex15
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Pex15(aa 1-309)
  • Entrez Gene
    PEX15 (a.k.a. YOL044W, PAS21)
  • Promoter T7
  • Tags / Fusion Proteins
    • prescission -GSS-FLAG-6xHis (C terminal on insert)
    • MBP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM251 was a gift from Andreas Martin (Addgene plasmid # 226895 ; http://n2t.net/addgene:226895 ; RRID:Addgene_226895)

  • For your References section:

    The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity. Castanzo DT, LaFrance B, Martin A. Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14970-14977. doi: 10.1073/pnas.1920109117. Epub 2020 Jun 15. 10.1073/pnas.1920109117 PubMed 32541053
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