pAM250
(Plasmid
#226894)
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PurposePex15(1-253)-PSP-6xHis-FLAG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 226894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-DUET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepex15
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SpeciesS. cerevisiae (budding yeast)
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MutationPex15(aa 1-253)
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Entrez GenePEX15 (a.k.a. YOL044W, PAS21)
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Tag
/ Fusion Protein
- prescission -GSS-FLAG-6xHis (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM250 was a gift from Andreas Martin (Addgene plasmid # 226894 ; http://n2t.net/addgene:226894 ; RRID:Addgene_226894) -
For your References section:
The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity. Castanzo DT, LaFrance B, Martin A. Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14970-14977. doi: 10.1073/pnas.1920109117. Epub 2020 Jun 15. 10.1073/pnas.1920109117 PubMed 32541053