EID1-pGex-2TK
(Plasmid #22689)

• Depositing Lab: William Kaelin
• Publication: Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-902.

Backbone

• Vector backbone: pGex2T
• Backbone manufacturer: Pharmacia
• Backbone size w/o insert (bp): 4938
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha, XL1 etc. for propagation of plasmid, BL21 or derivatives for protein expression
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E1A-like inhibitor of differentiation 1
• Alt name: EID1
• Species: H. sapiens (human)
• Insert Size (bp): 574
• Mutation: wt cDNA
• GenBank ID: NM_014335
• Entrez Gene: EID1 (a.k.a. PNAS-22, C15orf3, CRI1, EID-1, IRO45620, MGC138883, MGC138884, PTD014, RBP21)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: pGex5'
• 3′ sequencing primer: pGex3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry