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Addgene

pAM245
(Plasmid #226889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-DUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    msp1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    MSP1 (a.k.a. YGR028W, YTA4)
  • Promoter T7
  • Tag / Fusion Protein
    • His-Precission-PANN (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM245 was a gift from Andreas Martin (Addgene plasmid # 226889 ; http://n2t.net/addgene:226889 ; RRID:Addgene_226889)

  • For your References section:

    The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity. Castanzo DT, LaFrance B, Martin A. Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14970-14977. doi: 10.1073/pnas.1920109117. Epub 2020 Jun 15. 10.1073/pnas.1920109117 PubMed 32541053
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