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pET-NPR4 (SA binding domain)
(Plasmid #226885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 5752
  • Total vector size (bp) 6166
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    The Salicylic acid (SA) binding domain of NPR4
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    414
  • Mutation
    NPR4 SA-binding core (residues 373-516), with a short internal deletion of residues 450-455.
  • GenBank ID
    NM_118086.3
  • Entrez Gene
    NPR4 (a.k.a. AT4G19660, ATNPR4, NPR1-like protein 4, T16H5.20, T16H5_20)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-NPR4 (SA binding domain) was a gift from Ning Zheng (Addgene plasmid # 226885 ; http://n2t.net/addgene:226885 ; RRID:Addgene_226885)

  • For your References section:

    Structural basis of salicylic acid perception by Arabidopsis NPR proteins. Wang W, Withers J, Li H, Zwack PJ, Rusnac DV, Shi H, Liu L, Yan S, Hinds TR, Guttman M, Dong X, Zheng N. Nature. 2020 Oct;586(7828):311-316. doi: 10.1038/s41586-020-2596-y. Epub 2020 Aug 12. 10.1038/s41586-020-2596-y PubMed 32788727
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