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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)
(Plasmid #226884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226884 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 7119
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NaVAb
  • Species
    Aliarcobacter butzleri (strain RM4018)
  • Insert Size (bp)
    1914
  • Mutation
    R4A, N49K, L109A, M116V
  • GenBank ID
    ABV67998.1
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CGCGCGGAATTCATGAAAATCGAAGAAGGTAAACTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-Dual-MBP-NavAb (KAV/G94C/Q150C) was a gift from Ning Zheng (Addgene plasmid # 226884 ; http://n2t.net/addgene:226884 ; RRID:Addgene_226884)

  • For your References section:

    Resting-State Structure and Gating Mechanism of a Voltage-Gated Sodium Channel. Wisedchaisri G, Tonggu L, McCord E, Gamal El-Din TM, Wang L, Zheng N, Catterall WA. Cell. 2019 Aug 8;178(4):993-1003.e12. doi: 10.1016/j.cell.2019.06.031. Epub 2019 Jul 25. 10.1016/j.cell.2019.06.031 PubMed 31353218
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