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Addgene

pU6-GG-gRNA
(Plasmid #226860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MLM3636
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sp-gRNA
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Other
  • Promoter hU6

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: BsmBI (destroyed), 3' Cloning Site: BsmBI (destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-GG-gRNA was a gift from Alexis Komor (Addgene plasmid # 226860 ; http://n2t.net/addgene:226860 ; RRID:Addgene_226860)

  • For your References section:

    Genome editing with programmable base editors in human cells. Osgood NRB, Zawalick NM, Sawyer CB, Cowan QT, Gu S, Mawson SJ, Ranzau BL, Li L, Gymrek M, Goren A, Komor AC. Methods in Enzymology, February 5, 2025 10.1016/bs.mie.2025.01.001
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