DHIP1
(Bacterial strain
#226851)
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PurposeExpresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 226851 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DHIP1
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDH10β atpi:slr0214:gidB
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SpeciesE. coli
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Strain has resistance to streptomycin but not spectinomycin, use spectinomycin 100 µg/mL for plasmids with a streptomycin resistance cassette.
This strain methylates the first cytosine in the palindrome CGATCG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DHIP1 was a gift from David Nielsen (Addgene plasmid # 226851) -
For your References section:
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Kamoku C, Nielsen DR. Biotechnol Prog. 2025 Jan 23:e3518. doi: 10.1002/btpr.3518. 10.1002/btpr.3518 PubMed 39846505