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Addgene

pAH33
(Plasmid #226850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pR26-CMVconst
  • Backbone manufacturer
    Invitrogen (Life Technologies)
  • Backbone size w/o insert (bp) 7941
  • Total vector size (bp) 11834
  • Vector type
    Mouse Targeting, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Opa1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2880
  • Entrez Gene
    Opa1 (a.k.a. 1200011N24Rik, lilr3, mKIAA0567)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGGGATGCTAGCATGTGGCGAGCAGGTC
  • 3′ sequencing primer CCGCCTGCAGGTCACTTCTCCTGGTGAAGAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAH33 was a gift from Luke Chao (Addgene plasmid # 226850 ; http://n2t.net/addgene:226850 ; RRID:Addgene_226850)

  • For your References section:

    In situ architecture of Opa1-dependent mitochondrial cristae remodeling. Fry MY, Navarro PP, Hakim P, Ananda VY, Qin X, Landoni JC, Rath S, Inde Z, Lugo CM, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH. EMBO J. 2024 Feb;43(3):391-413. doi: 10.1038/s44318-024-00027-2. Epub 2024 Jan 15. 10.1038/s44318-024-00027-2 PubMed 38225406
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