NXPE1_pcDNA3.4-TOPO
(Plasmid #226841)

• Purpose: Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
• Depositing Lab: Bert Vogelstein
• Publication: NXPE1 Protein Producing Plasmid (unpublished)

Backbone

• Vector backbone: pcDNA3.4-TOPO
• Backbone manufacturer: Thermo Fisher
• Total vector size (bp): 7576
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NXPE1
• Alt name: FAM55A
• Species: H. sapiens (human)
• Mutation: Codon optimized for expression in human cells
• GenBank ID: NM_152315.5
• Entrez Gene: NXPE1 (a.k.a. FAM55A)
• Tag / Fusion Protein:
  • x6 HIS and an IL2 secretion signal both N terminal (N terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: CGC AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: CAA CAT AGT TAA GAA TAC CAG TC

Resource Information

• Supplemental Documents:
  • 24AAW5NC_3775442_NW03_N-term-His.gb
• A portion of this plasmid was derived from a plasmid made by: GeneArt produced it for us at our request and by our design.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry