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Addgene

pcDNA3.1-AntiHer2-LC122-TAG
(Plasmid #226839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226839 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 8483
  • Vector type
    Mammalian Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    anti-HER2 light chain
  • Alt name
    Trastuzumab light chain
  • Species
    H. sapiens (human)
  • Mutation
    amino acid 122 of light chain mutated to TAG

Gene/Insert 2

  • Gene/Insert name
    anti-HER2 heavy chain
  • Alt name
    Trastuzumab heavy chain
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-AntiHer2-LC122-TAG was a gift from Abhishek Chatterjee (Addgene plasmid # 226839 ; http://n2t.net/addgene:226839 ; RRID:Addgene_226839)

  • For your References section:

    A Genetically Encoded Photocaged Cysteine for Facile Site-Specific Introduction of Conjugation-Ready Thiol Residues in Antibodies. Osgood AO, Roy SJS, Koo D, Gu R, Chatterjee A. Bioconjug Chem. 2024 Apr 17;35(4):457-464. doi: 10.1021/acs.bioconjchem.3c00513. Epub 2024 Mar 28. 10.1021/acs.bioconjchem.3c00513 PubMed 38548654
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