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Addgene

pISClsp3-reRNA
(Plasmid #226824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXMJ19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ISClsp3 and its corresponding guide RNA
  • Species
    Clostridiales bacterium AM23-16LB

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISClsp3-reRNA was a gift from Sheng Yang (Addgene plasmid # 226824 ; http://n2t.net/addgene:226824 ; RRID:Addgene_226824)

  • For your References section:

    Implementation of RAGATH RNA-associated DNA Endonucleases as Genome Editing Tool in Escherichia coli. Zhou X, Yang S, Sun B, Dong F, Yin M, Jiang Y, Huang Z, Yang S. Biotechnol J. 2025 Mar;20(3):e70005. doi: 10.1002/biot.70005. 10.1002/biot.70005 PubMed 40111000
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