pCMK195
(Plasmid
#226818)
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Purpose(Empty Backbone) Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 5356
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.10.611283 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMK195 was a gift from David Nielsen (Addgene plasmid # 226818 ; http://n2t.net/addgene:226818 ; RRID:Addgene_226818) -
For your References section:
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Kamoku C, Nielsen DR. Biotechnol Prog. 2025 Jan 23:e3518. doi: 10.1002/btpr.3518. 10.1002/btpr.3518 PubMed 39846505