pInducer20 2xHA LTB4R
(Plasmid
#226810)
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PurposeTet-inducible lentiviral vector for 2xHA LTB4R expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226810 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepInducer20
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Backbone manufacturerAddgene #44012
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLTB4R
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SpeciesH. sapiens (human)
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Insert Size (bp)1146
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MutationSequence optimized from NP_858043.1
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Entrez GeneLTB4R (a.k.a. BLT1, BLTR, CMKRL1, GPR16, LTB4R1, LTBR1, P2RY7, P2Y7)
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Tag
/ Fusion Protein
- 2xHA tag followed by a GDPPVAT linker (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer LNCX_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pInducer20 2xHA LTB4R was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 226810 ; http://n2t.net/addgene:226810 ; RRID:Addgene_226810) -
For your References section:
GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates Epithelial Proliferation, Migration, and Cellular Respiration. Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, Iglesias-Bartolome R. J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 10.1016/j.jid.2023.12.002. Epub 2023 Dec 14. 10.1016/j.jid.2023.12.002 PubMed 38103827