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Addgene

pENTR1A 2xHA HCAR3
(Plasmid #226807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226807 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Addgene #17398
  • Vector type
    Gateway Entry

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HCAR3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1251
  • Mutation
    Sequence optimized from NP_006009.2
  • Entrez Gene
    HCAR3 (a.k.a. GPR109B, HCA3, HM74, PUMAG, Puma-g)
  • Tag / Fusion Protein
    • 2xHA tag followed by a GDPPVAT linker (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ENTRforw
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1A 2xHA HCAR3 was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 226807 ; http://n2t.net/addgene:226807 ; RRID:Addgene_226807)

  • For your References section:

    GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates Epithelial Proliferation, Migration, and Cellular Respiration. Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, Iglesias-Bartolome R. J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 10.1016/j.jid.2023.12.002. Epub 2023 Dec 14. 10.1016/j.jid.2023.12.002 PubMed 38103827
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