pCMK83
(Plasmid
#226793)
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PurposeIntegrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A
- Backbone size w/o insert (bp) 3446
- Total vector size (bp) 4784
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameslr0214
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SpeciesSynechocystis sp. PCC 6803
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Insert Size (bp)1338
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Entrez Geneslr0214 (a.k.a. SYNGTS_2246)
- Promoter Tetracycline resistance cassette promoter (constitutive)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.10.611283 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMK83 was a gift from David Nielsen (Addgene plasmid # 226793 ; http://n2t.net/addgene:226793 ; RRID:Addgene_226793) -
For your References section:
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Kamoku C, Nielsen DR. Biotechnol Prog. 2025 Jan 23:e3518. doi: 10.1002/btpr.3518. 10.1002/btpr.3518 PubMed 39846505