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Addgene

pQE30-DARPin-E11
(Plasmid #226784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DARPin E11
  • Species
    Synthetic
  • Insert Size (bp)
    546
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • FLAG (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andreas Plückthun (UZH)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-DARPin-E11 was a gift from Sebastian Mathea (Addgene plasmid # 226784 ; http://n2t.net/addgene:226784 ; RRID:Addgene_226784)

  • For your References section:

    A designed ankyrin-repeat protein that targets Parkinson's disease-associated LRRK2. Dederer V, Sanz Murillo M, Karasmanis EP, Hatch KS, Chatterjee D, Preuss F, Abdul Azeez KR, Nguyen LV, Galicia C, Dreier B, Pluckthun A, Versees W, Mathea S, Leschziner AE, Reck-Peterson SL, Knapp S. J Biol Chem. 2024 Jul;300(7):107469. doi: 10.1016/j.jbc.2024.107469. Epub 2024 Jun 12. 10.1016/j.jbc.2024.107469 PubMed 38876305
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