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Addgene

pFB-EPCP1
(Plasmid #226783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-6HZB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Leucine-rich repeat kinase 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3854
  • Mutation
    Amino acids deleted 1-1326
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Tag / Fusion Protein
    • His-Z-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-EPCP1 was a gift from Sebastian Mathea (Addgene plasmid # 226783 ; http://n2t.net/addgene:226783 ; RRID:Addgene_226783)

  • For your References section:

    A designed ankyrin-repeat protein that targets Parkinson's disease-associated LRRK2. Dederer V, Sanz Murillo M, Karasmanis EP, Hatch KS, Chatterjee D, Preuss F, Abdul Azeez KR, Nguyen LV, Galicia C, Dreier B, Pluckthun A, Versees W, Mathea S, Leschziner AE, Reck-Peterson SL, Knapp S. J Biol Chem. 2024 Jul;300(7):107469. doi: 10.1016/j.jbc.2024.107469. Epub 2024 Jun 12. 10.1016/j.jbc.2024.107469 PubMed 38876305
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