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Addgene

pB6-ROSA26::lacZ
(Plasmid #22678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22678 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    SAbetageolox2DTA
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    beta-geo
  • Insert Size (bp)
    3000
  • Mutation
    Targeting vector for the Gt(ROSA26) locus. Beta-geo insert.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Upon NGS sequencing, we discovered that an incorrect clone had been deposited. The author of this paper is no longer in the laboratory, and we do not have the clones to re-deposit. We note that this plasmid is a construct distinct from the ROSA26-CAG-lsl-tauLacZ reporter construct described in Nam and Benezra, 2009. If the user seeks B6J ROSA26 locus homology arms for targeting vector construction, we recommend plasmids deposited at http://www.addgene.org/browse/article/28203395/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB6-ROSA26::lacZ was a gift from Robert Benezra (Addgene plasmid # 22678)

  • For your References section:

    High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442
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