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pKC50
(Plasmid #226778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    GENEVA Biotech
  • Backbone size w/o insert (bp) 3641
  • Total vector size (bp) 8699
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drosophila unc-104 (V6M)
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    5000
  • Mutation
    changed Valine 6 to Methionine
  • Entrez Gene
    unc-104 (a.k.a. Dmel_CG8566, CG8566, DUNC104, DUnc-104, DmCG8566, DmDUnc104, DmKlp53D, Dmel\CG8566, Dunc104, Imac, KIF 1B, KIF1A, KIF1A/1B, Kif1a, Klp53D, Unc-104, Unc104, bris, imac, kif1a, unc104)
  • Tag / Fusion Protein
    • sfGFP and 2xStrepII (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKC50 was a gift from Kyoko Chiba (Addgene plasmid # 226778 ; http://n2t.net/addgene:226778 ; RRID:Addgene_226778)

  • For your References section:

    The FHA domain is essential for the autoinhibition of KIF1A/UNC-104. Niwa S, Watanabe T, Chiba K. J Cell Sci. 2024 Sep 6:jcs.262017. doi: 10.1242/jcs.262017. 10.1242/jcs.262017 PubMed 39239883
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