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pCMV6-CNDP2-E166L
(Plasmid #226721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226721 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV6-Entry
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CNDP2
  • Species
    M. musculus (mouse)
  • Mutation
    E166L
  • Entrez Gene
    Cndp2 (a.k.a. 0610010E05Rik, Cn2, Dip-2, Pep-1, Pep1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SgfI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer VP1.5
  • 3′ sequencing primer XL39
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a 31bp deletion in the SV40 promoter. This deletion is not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-CNDP2-E166L was a gift from Jonathan Long (Addgene plasmid # 226721 ; http://n2t.net/addgene:226721 ; RRID:Addgene_226721)

  • For your References section:

    A beta-hydroxybutyrate shunt pathway generates anti-obesity ketone metabolites. Moya-Garzon MD, Wang M, Li VL, Lyu X, Wei W, Tung AS, Raun SH, Zhao M, Coassolo L, Islam H, Oliveira B, Dai Y, Spaas J, Delgado-Gonzalez A, Donoso K, Alvarez-Buylla A, Franco-Montalban F, Letian A, Ward CP, Liu L, Svensson KJ, Goldberg EL, Gardner CD, Little JP, Banik SM, Xu Y, Long JZ. Cell. 2025 Jan 9;188(1):175-186.e20. doi: 10.1016/j.cell.2024.10.032. Epub 2024 Nov 12. 10.1016/j.cell.2024.10.032 PubMed 39536746
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