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pCS2-hcar1-4-mKate2
(Plasmid #226712)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226712 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5754
  • Vector type
    Unspecified ; Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hcar1-4
  • Species
    danio rerio
  • Insert Size (bp)
    1239
  • GenBank ID
    ENSDARG00000087084
  • Promoter SP6
  • Tag / Fusion Protein
    • mKate2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer atttaggtgacactataga
  • 3′ sequencing primer tgccctccatgtacagcttc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-hcar1-4-mKate2 was a gift from Philipp Niethammer (Addgene plasmid # 226712 ; http://n2t.net/addgene:226712 ; RRID:Addgene_226712)

  • For your References section:

    Oxoeicosanoid signaling mediates early antimicrobial defense in zebrafish. Ma Y, Hui KL, Gelashvili Z, Niethammer P. Cell Rep. 2023 Jan 31;42(1):111974. doi: 10.1016/j.celrep.2022.111974. Epub 2023 Jan 10. 10.1016/j.celrep.2022.111974 PubMed 36640321
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