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Addgene

pRGEB32-CrU6-2-35S
(Plasmid #226706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia1300
  • Vector type
    CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CrU6-2
  • Species
    C. richardii

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.10.588889 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGEB32-CrU6-2-35S was a gift from Fenglin Deng (Addgene plasmid # 226706 ; http://n2t.net/addgene:226706 ; RRID:Addgene_226706)

  • For your References section:

    Efficient gene editing of a model fern species through gametophyte-based transformation. Jiang W, Deng F, Babla M, Chen C, Yang D, Tong T, Qin Y, Chen G, Marchant B, Soltis P, Soltis DE, Zeng F, Chen ZH. Plant Physiol. 2024 Sep 12:kiae473. doi: 10.1093/plphys/kiae473. 10.1093/plphys/kiae473 PubMed 39268871
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