pAM102
(Plasmid
#226694)
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Purpose6xHis-Sumo-Ub-Ub-Ub-Ub
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUB
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Alt namelinear tetra Ubiquitin
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneUBI4 (a.k.a. YLL039C, SCD2, UB14)
- Promoter T7
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Tag
/ Fusion Protein
- His-SUMO (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM102 was a gift from Andreas Martin (Addgene plasmid # 226694 ; http://n2t.net/addgene:226694 ; RRID:Addgene_226694) -
For your References section:
The 26S Proteasome Utilizes a Kinetic Gateway to Prioritize Substrate Degradation. Bard JAM, Bashore C, Dong KC, Martin A. Cell. 2019 Apr 4;177(2):286-298.e15. doi: 10.1016/j.cell.2019.02.031. Epub 2019 Mar 28. 10.1016/j.cell.2019.02.031 PubMed 30929903