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pAM89
(Plasmid #226681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226681 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    rpt1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPT1 (a.k.a. YKL145W, CIM5, YTA3)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Rpt2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPT2 (a.k.a. YDL007W, YHS4, YTA5)

Gene/Insert 3

  • Gene/Insert name
    Rpt3
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPT3 (a.k.a. YDR394W, YNT1, YTA2)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Gene/Insert 4

  • Gene/Insert name
    Rpt4
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPT4 (a.k.a. YOR259C, CRL13, PCS1, SUG2)

Gene/Insert 5

  • Gene/Insert name
    Rpt5
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Q49TAG
  • Entrez Gene
    RPT5 (a.k.a. YOR117W, YTA1)

Gene/Insert 6

  • Gene/Insert name
    Rpt6
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPT6 (a.k.a. YGL048C, CIM3, CRL3, SCB68, SUG1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM89 was a gift from Andreas Martin (Addgene plasmid # 226681 ; http://n2t.net/addgene:226681 ; RRID:Addgene_226681)

  • For your References section:

    The 26S Proteasome Utilizes a Kinetic Gateway to Prioritize Substrate Degradation. Bard JAM, Bashore C, Dong KC, Martin A. Cell. 2019 Apr 4;177(2):286-298.e15. doi: 10.1016/j.cell.2019.02.031. Epub 2019 Mar 28. 10.1016/j.cell.2019.02.031 PubMed 30929903
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