pTBL3328 PB-sfGFP-WPRE-target
(Plasmid
#226676)
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PurposePiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMTK0 PiggyBac cargo backbone
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Backbone manufacturerHana El-Samad Lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP-WPRE-site3target
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SpeciesSynthetic
- Promoter EF1alpha
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer ColE1 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJohn Dueber's lab (sfGFP was obtained from the MoClo Yeast Toolkit, Kit #1000000061), and Hana El-Samad (Mammalian Toolkit, Kit #1000000180).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.05.467507 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTBL3328 PB-sfGFP-WPRE-target was a gift from Chang Liu (Addgene plasmid # 226676 ; http://n2t.net/addgene:226676 ; RRID:Addgene_226676) -
For your References section:
Open-ended molecular recording of sequential cellular events into DNA. Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC. bioRxiv 2021.11.05.467507 10.1101/2021.11.05.467507