pTBL3478 PB-PEmax-mTagBFP2
(Plasmid
#226664)
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PurposePiggyBac cargo vector encoding PEmax and mTagBFP2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMTK0 PiggyBac cargo backbone
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Backbone manufacturerHana El-Samad Lab
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Vector typeMammalian Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePEmax
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SpeciesSynthetic
- Promoter CAG
Cloning Information for Gene/Insert 1
- Cloning method Golden Gate
- 5′ sequencing primer CAG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemTagBFP2
- Promoter UBC
Cloning Information for Gene/Insert 2
- Cloning method Golden Gate
- 5′ sequencing primer UBC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHana El-Samad (Mammalian Toolkit, Kit #1000000180).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.05.467507 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTBL3478 PB-PEmax-mTagBFP2 was a gift from Chang Liu (Addgene plasmid # 226664 ; http://n2t.net/addgene:226664 ; RRID:Addgene_226664) -
For your References section:
Open-ended molecular recording of sequential cellular events into DNA. Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC. bioRxiv 2021.11.05.467507 10.1101/2021.11.05.467507