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Addgene

pCMK74
(Plasmid #226657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAV1K-T5-gfp
  • Backbone manufacturer
    Ichiro Matsumura, Addgene plasmid #26702
  • Backbone size (bp) 2731
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Grow at 30°C in E. coli, grow at 37°C in Synechococcus sp. PCC 7002
Grow in Synechococcus sp. PCC 7002 with Kanamycin 100 µg/mL

For increased transformation into PCC 7002, miniprep this plasmid from E.coli strain DHIP1.
DHIP1 deposited under the following paper:

Kamoku C, Nielsen DR. Highly Iterated Palindrome 1 (HIP1) sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Published online September 10, 2024:2024.09.10.611283. doi:10.1101/2024.09.10.611283

Please visit https://doi.org/10.1101/2024.07.31.606084 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMK74 was a gift from David Nielsen (Addgene plasmid # 226657 ; http://n2t.net/addgene:226657 ; RRID:Addgene_226657)
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