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Addgene

pAM231
(Plasmid #226653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p97
  • Species
    H. sapiens (human)
  • Mutation
    T262A
  • Entrez Gene
    VCP (a.k.a. CDC48, FTDALS6, TERA, p97)
  • Tags / Fusion Proteins
    • His (C terminal on insert)
    • ybbR (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM231 was a gift from Andreas Martin (Addgene plasmid # 226653 ; http://n2t.net/addgene:226653 ; RRID:Addgene_226653)

  • For your References section:

    Multisystem Proteinopathy Mutations in VCP/p97 Increase NPLOC4.UFD1L Binding and Substrate Processing. Blythe EE, Gates SN, Deshaies RJ, Martin A. Structure. 2019 Dec 3;27(12):1820-1829.e4. doi: 10.1016/j.str.2019.09.011. Epub 2019 Oct 14. 10.1016/j.str.2019.09.011 PubMed 31623962
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