pAM224
(Plasmid
#226647)
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PurposepET24b-p97-T262A-His6
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep97
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SpeciesH. sapiens (human)
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MutationT262A
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Entrez GeneVCP (a.k.a. CDC48, FTDALS6, TERA, p97)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM224 was a gift from Andreas Martin (Addgene plasmid # 226647 ; http://n2t.net/addgene:226647 ; RRID:Addgene_226647) -
For your References section:
Multisystem Proteinopathy Mutations in VCP/p97 Increase NPLOC4.UFD1L Binding and Substrate Processing. Blythe EE, Gates SN, Deshaies RJ, Martin A. Structure. 2019 Dec 3;27(12):1820-1829.e4. doi: 10.1016/j.str.2019.09.011. Epub 2019 Oct 14. 10.1016/j.str.2019.09.011 PubMed 31623962