pAM238
(Plasmid
#226639)
-
PurposeS. cerevisiae ubiquitin
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 226639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneunknown
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameUbiquitin
-
SpeciesS. cerevisiae (budding yeast)
-
Entrez GeneUBI4 (a.k.a. YLL039C, SCD2, UB14)
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promotor (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAM238 was a gift from Andreas Martin (Addgene plasmid # 226639 ; http://n2t.net/addgene:226639 ; RRID:Addgene_226639) -
For your References section:
Complete subunit architecture of the proteasome regulatory particle. Lander GC, Estrin E, Matyskiela ME, Bashore C, Nogales E, Martin A. Nature. 2012 Jan 11;482(7384):186-91. doi: 10.1038/nature10774. 10.1038/nature10774 PubMed 22237024