pDL1531
(Plasmid
#226637)
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PurposeThis plasmid is for correcting the nonsense mutation in the nrsB gene of several ICP1 isoaltes.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD18
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUpstream sequence of nrsB (only 338bp total)
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SpeciesICP1_2001 bacteriophage
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.17.599363 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDL1531 was a gift from Andrew Camilli (Addgene plasmid # 226637 ; http://n2t.net/addgene:226637 ; RRID:Addgene_226637) -
For your References section:
A Vibrio cholerae anti-phage system depletes nicotinamide adenine dinucleotide to restrict virulent bacteriophages. Woldetsadik YA, Lazinski DW, Camilli A. mBio. 2024 Oct 8:e0245724. doi: 10.1128/mbio.02457-24. 10.1128/mbio.02457-24 PubMed 39377576