PM-ExRai-PKNAR(T/A)
(Plasmid
#226634)
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PurposeNegative-control mutant for PM-ExRai-PKNAR biosensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePM-ExRai-PKNAR(T/A)
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MutationPhosphorylatable Thr of PM-ExRai-PKNAR mutated to Ala
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byParent plasmid Exrai-AKAR2 was a gift from Jin Zhang lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PM-ExRai-PKNAR(T/A) was a gift from Ju Chen (Addgene plasmid # 226634 ; http://n2t.net/addgene:226634 ; RRID:Addgene_226634) -
For your References section:
Illuminating understudied kinases: a generalizable biosensor development method applied to protein kinase N. Bogomolovas J, Chen J. Commun Biol. 2025 Jan 22;8(1):109. doi: 10.1038/s42003-025-07510-4. 10.1038/s42003-025-07510-4 PubMed 39843538