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Addgene

pCKC017
(Plasmid #226622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV Blast Dest (Addgene #17451)
  • Backbone size w/o insert (bp) 8100
  • Modifications to backbone
    Ampicillin resistance gene was replaced with kanamycin resistance, the CMV promoter sequence was extended, and a a pPGK-sfGFP-P2A-puroR cassette was added
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Terminal deoxynucleotidyl transferase
  • Alt name
    TdT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2167
  • Mutation
    synonymous mutation in D2, synonymous mutation in V414, endogenous HEK3 sequence added downstream of TdT gene
  • Entrez Gene
    DNTT (a.k.a. TDT)
  • Promoter CMV

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Chang Liu's lab (Addgene plasmid #126450)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has also been grown in Invitrogen One Shot Stbl3 chemically competent E. coli.

Please visit https://doi.org/10.1101/2024.06.11.598561 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCKC017 was a gift from Chang Liu (Addgene plasmid # 226622 ; http://n2t.net/addgene:226622 ; RRID:Addgene_226622)

  • For your References section:

    A massively parallel in vivo assay of TdT mutants yields variants with altered nucleotide insertion biases. Carlson CK, Loveless TB, Milisavljevic M, Kelly PI, Mills JH, Tyo KEJ, Liu CC. bioRxiv [Preprint]. 2024 Jun 11:2024.06.11.598561. doi: 10.1101/2024.06.11.598561. 10.1101/2024.06.11.598561 PubMed 38915690
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