pCDHblast MCSNard OST-LMNA
(Plasmid #22661)

• Depositing Lab: Tom Misteli
• Publication: Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Backbone

• Vector backbone: pCDHblast
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lamin A
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Tag / Fusion Protein:
  • Onestrep Tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI, XhoI, NotI, SwaI, BglII, AsuII (not destroyed)
• 5′ sequencing primer: AAATGGGCGGTAGGCGTGT
• 3′ sequencing primer: TCTCTAGGCACCCGTTCAAT

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry