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Addgene

EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
(Plasmid #226590)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226590 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFB
  • Backbone manufacturer
    Virovek
  • Backbone size w/o insert (bp) 4825
  • Total vector size (bp) 9030
  • Modifications to backbone
    N/A
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4205
  • Tag / Fusion Protein
    • Fusion Red (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GACTGAAGTTAGGCCAGCT
  • 3′ sequencing primer GACGTACACAACCAGTCTG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Chemical and genetic engineering of selective ion channel-ligand interactions. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM. Science. 2011 Sep 2;333(6047):1292-6. 10.1126/science.1206606 PubMed 21885782

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.27.586699 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed was a gift from D. James Surmeier (Addgene plasmid # 226590 ; http://n2t.net/addgene:226590 ; RRID:Addgene_226590)