EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
(Plasmid #226590)

• Purpose: Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
• Depositing Lab: D. James Surmeier
• Publication: Tonic dendritic GABA release by substantia nigra dopaminergic neurons (unpublished)

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Backbone

• Vector backbone: pFB
• Backbone manufacturer: Virovek
• Backbone size w/o insert (bp): 4825
• Total vector size (bp): 9030
• Modifications to backbone: N/A
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
• Species: H. sapiens (human)
• Insert Size (bp): 4205
• Tag / Fusion Protein:
  • Fusion Red (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GACTGAAGTTAGGCCAGCT
• 3′ sequencing primer: GACGTACACAACCAGTCTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Chemical and genetic engineering of selective ion channel-ligand interactions. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM. Science. 2011 Sep 2;333(6047):1292-6. 10.1126/science.1206606 PubMed 21885782

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.27.586699 for bioRxiv preprint.