EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
(Plasmid
#226590)
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PurposePharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFB
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Backbone manufacturerVirovek
- Backbone size w/o insert (bp) 4825
- Total vector size (bp) 9030
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Modifications to backboneN/A
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
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SpeciesH. sapiens (human)
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Insert Size (bp)4205
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Tag
/ Fusion Protein
- Fusion Red (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GACTGAAGTTAGGCCAGCT
- 3′ sequencing primer GACGTACACAACCAGTCTG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byChemical and genetic engineering of selective ion channel-ligand interactions. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM. Science. 2011 Sep 2;333(6047):1292-6. 10.1126/science.1206606 PubMed 21885782
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.27.586699 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed was a gift from D. James Surmeier (Addgene plasmid # 226590 ; http://n2t.net/addgene:226590 ; RRID:Addgene_226590)