mNeonGreen-Sec31A
(Plasmid
#226582)
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PurposeEncodes Sec31A protein labeled with mNeonGreen
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemNeonGreen-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec31A (Protein transport protein)
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SpeciesH. sapiens (human)
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Entrez GeneSEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
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Tag
/ Fusion Protein
- mNeonGreen (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mNeonGreen-Sec31A was a gift from Gia Voeltz (Addgene plasmid # 226582 ; http://n2t.net/addgene:226582 ; RRID:Addgene_226582) -
For your References section:
Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport. Westrate LM, Hoyer MJ, Nash MJ, Voeltz GK. J Cell Sci. 2020 Jul 24;133(14). pii: jcs.239814. doi: 10.1242/jcs.239814. 10.1242/jcs.239814 PubMed 32616562