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siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)
(Plasmid #226578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mNeonGreen-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SigmaR1 (Sigma-1 Receptor)
  • Species
    H. sapiens (human)
  • Mutation
    F196A, L199A, F200A, L203A, Y206A, L210A, L214A, Y217A
  • Entrez Gene
    SIGMAR1 (a.k.a. ALS16, DSMA2, OPRS1, SIG-1R, SR-BP, SR-BP1, SRBP, hSigmaR1, sigma1R)
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A) was a gift from Gia Voeltz (Addgene plasmid # 226578 ; http://n2t.net/addgene:226578 ; RRID:Addgene_226578)

  • For your References section:

    SigmaR1 shapes rough endoplasmic reticulum membrane sheets. Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, Voeltz GK. Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 10.1016/j.devcel.2024.06.005. 10.1016/j.devcel.2024.06.005 PubMed 38971154
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