pC2.4
(Plasmid
#226557)
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PurposeNATURA gene encoding plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226557 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac
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Vector typeMammalian Expression, Luciferase ; Piggybac
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNATURA reporter gene
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SpeciesSynthetic
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Insert Size (bp)6700
- Promoter EF1alpha
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC2.4 was a gift from Dave Wee (Addgene plasmid # 226557 ; http://n2t.net/addgene:226557 ; RRID:Addgene_226557) -
For your References section:
Unveiling Sequence-Agnostic Mixed-Chemical Modification Patterns for SpliceSwitching Oligonucleotides using the NATURA platform. Tommaso T, Agarwal T, Cher WY, Ow JR, Chew AK, Qian Sun PY, Reddy Gurrampati RS, Lu H, Naidu P, Ng HK, Le Guezennec X, Ng SY, Lakshmanan M, Ernesto G, Wee KB. Molecular Therapy: Nucleic Acid (2025) 10.1016/j.omtn.2024.102422